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ORIGINAL ARTICLE
Year : 2020  |  Volume : 7  |  Issue : 3  |  Page : 169-173

Assessment of the low-speed centrifugation concept modified in the release of fibroblast growth factor-2 in Saudi healthy patient


1 Aseer Dental Center, Saudi Ministry of Health, Aseer Region, Riyadh City, Saudi Arabia
2 Department Periodontal, Dental Center, Saudi Ministry of Health, King Saud Medical City, Riyadh City, Saudi Arabia
3 Department of Periodontal, Dental School, Riyadh Elm University, Riyadh City, Saudi Arabia
4 Eastman Institute for Oral Health, Rochester, New York, USA, USA
5 School of Dentistry, Tishk International University, Erbil Kurdistan, Iraq
6 Department of Endodontics and Periodontics, Dental School, Riyadh Elm University, Riyadh City, Saudi Arabia

Correspondence Address:
Dr. Abdulrahman Alshehri
Ministry of Health, Aseer Region
Saudi Arabia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/sjos.SJOralSci_97_19

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Introduction: Alteration of the centrifugation time and protocol may impact the release of platelet-rich fibrin (PRF) scaffolds. The current study purpose was to investigate the released levels of fibroblast growth factor-2 (FGF-2) in standard PRF (S-PRF) and low-speed centrifugation concept known as advanced PRF (A-PRF and A-PRF+). Measurements were done at five times interval over 42 days. The aim was to assess the FGF release and the relation between initial platelet counts and the concentrations of FGF-2 release using the following technique: (1) S-PRF, (2) A-PRF, and (3) A-PRF+. Materials and Methods: Twenty-four blood samples were taken from eight random Saudi Arabian national healthy subjects enrolled in the investigation. Blood samples were processed using S-PRF, A-PRF, and A-PRF+ centrifugation protocols. Protein quantification was performed using enzyme-linked immunosorbent assay at 1, 7, 14, 28, and 42-day intervals. Results and Discussion: A statistically significant difference in the mean of FGF-2 measurement between protocols at the 7th day where both S-PRF and A-PRF were significantly higher than A-PRF+ (P < 0.012). Initial platelets' significant count for S-PRF, A-PRF, and A-PRF+ was on day 1, day 7, and 7th day, respectively. Both protocols S-PRF and A-PRF yielded significantly higher release of the FGF-2 when compared to A-PRF+ in Saudi healthy subjects.


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